Zhengzhou Weier-elisa kit detection technology
First, ELISA test service introduction
[customer provided]
The following conditions should be noted when sending the specimen:
â—‡ 1, the specimen number; 2, the measured items; 3, whether to do the double hole; 3, contact information; 4, whether the specimen is sent back after the experiment.
The experimental requirements include: serum, plasma, urine, pleural and ascites, cerebrospinal fluid, cell culture supernatant, etc.
ã€Precautions】
Before collecting the specimen, the customer must know whether the ingredients to be tested are stable enough.
Specimens that were tested on the day after collection were stored at 4 °C for use.
Specimens that need to be collected periodically for special reasons need to be stored in time and stored at -20 °C or -70 °C, and avoid repeated freezing and thawing.
Specimens can be stored for 48 hours at 2-8 °C and for 1 month at -20 °C. It can be stored for 6 months at -70 °C.
Some hormone samples require the addition of aprotinin.
Note: For customers in the field, please contact our technical staff to confirm that they can communicate with them before mailing the specimen.
For local customers in Nanjing, please contact our technical staff to confirm that they are communicating well. We provide free on-site sampling service.
[Professional technology] The Senbega Bio-Lab is cooperating with many famous laboratories at home and abroad. Each technician has the skills of elisa kit testing, and has many years of experience in overseas study to improve the test data. Reliability.
[Short service period] 3-5 days, hand over the test report to the customer in a short time
ã€Charges】
If the customer provides the elisa kit itself, please contact our technical staff for the fee.
If you purchase our elisa kit, you can enjoy free test services.
[Company provided]
Experimental procedures, experimental methods, reagents, instruments, relevant data, etc., and ensure the accuracy of the experimental results.
Second, the specimen processing requirements:
Before collecting the specimen, it must be clear whether the ingredients to be tested are stable enough. Specimens that were tested on the day after collection should be stored at 4 °C. If there are special reasons, the specimens should be collected periodically, and the specimens should be stored in time and stored at -20 °C or -70 °C. Avoid repeated freezing and thawing. Specimens can be stored for 48 hours at 2 -8 °C and for 1 month at -20 °C. -70 degrees for 6 months. Some hormone samples require the addition of aprotinin.
液体 Liquid specimen:
Specimens must be liquid and free of sediment. Including serum, plasma, urine, pleural and ascites, cerebrospinal fluid, cell culture supernatant, tissue homogenate and the like. 1 ml of whole blood gives 0.5 ml of serum or plasma. Collection volume per specimen = 100ul × detection type. Ask the salesperson for instructions before taking the material.
â—‡ Serum:
The blood is naturally coagulated at room temperature for 10-20 minutes and then centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant. If a precipitate forms, it should be centrifuged again.
血浆 Plasma:
EDTA, sodium citrate or heparin should be selected as an anticoagulant according to the requirements of the kit. Add 10% (v/v) anticoagulant (0.1M sodium citrate or 1% heparin or 2.0% EDTA.Na2). After 20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms, it should be centrifuged again.
å°¿ Urine, chest and ascites, cerebrospinal fluid:
Collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms, it should be centrifuged again.
â—‡ Cell culture supernatant:
When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When measuring the components in the cells, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
â—‡ Organizational specimens:
After cutting the specimen, weigh the weight. Add a certain amount of PBS, and add 1 μg/L protease inhibitor or 50 U/ml Aprotinin (Aprotinin) to the buffer. The specimen was homogenized by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant at -20 degrees or -70 degrees and, if necessary, concentrate and dry the sample. One part of the package is to be tested, and the rest is frozen for use.
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