Insulin (INS) enzyme-linked immunosorbent assay (ELISA) kit instruction manual

This reagent is for research use only: This kit is used to determine the insulin (INS) content in rat serum, plasma, cell supernatant and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the insulin level (INS) in the specimen. The microplate is coated with purified rat insulin (INS) antibody to prepare a solid phase antibody, which is sequentially added to the microcapsule of the coated monoclonal antibody (INS), and then combined with HRP-labeled insulin (INS) antibody to form an antibody. - Antigen-enzyme-labeled antibody complex, after thorough washing, was added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with insulin (INS) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the rat (INS) concentration in the sample was calculated from a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 18mU / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C preservation Reagent B liquid 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation

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