**Detection Range:** 10 ng/L – 320 ng/L
**Purpose of Use:** This kit is designed to quantitatively measure the level of midkine (MK) in human serum, plasma, and other related biological samples. It provides a reliable and accurate method for detecting MK concentrations, which can be useful in clinical research and diagnostic applications.
**Experimental Principle:** The kit employs a double-antibody sandwich ELISA method to determine the concentration of midkine (MK) in the sample. A microtiter plate is pre-coated with a purified anti-MK antibody, forming a solid-phase capture antibody. The sample is then added, allowing MK to bind to the immobilized antibody. Following this, an HRP-conjugated anti-MK antibody is introduced, forming a complex of antibody-antigen-enzyme-labeled antibody. After thorough washing to remove unbound components, the substrate TMB is added. Under the catalytic action of HRP, TMB turns blue, and upon adding the stop solution, it changes to yellow. The intensity of the color is directly proportional to the concentration of MK in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the MK concentration is determined by comparing the results to a standard curve.
**Kit Composition:**
1. 30× Washing Solution – 20 ml × 1 bottle
2. Enzyme Standard Reagent – 6 ml × 1 bottle
3. Enzyme-Labeled Coating Plate – 12 wells × 8 strips
4. Sample Diluent – 6 ml × 1 bottle
5. Color Reagent A – 6 ml × 1 bottle
6. Color Reagent B – 6 ml × 1 bottle
7. Stop Solution – 6 ml × 1 bottle
8. Standard Product (640 ng/L) – 0.5 ml × 1 bottle
9. Standard Dilutions – 1.5 ml × 1 bottle
10. Instructions – 1 booklet
11. Sealing Film – 2 sheets
12. Seal Bag – 1 bag
This comprehensive kit ensures high specificity and sensitivity for midkine detection, making it suitable for both research and clinical settings. Proper storage and handling are essential to maintain the integrity and performance of the reagents.
Nail Polish Gel Remover
Nail polish remover is a product specially designed to remove nail polish. It belongs to the nail polish category and can remove nail polish effectively and quickly.
The specific components are some organic matter, is the relationship between solute and solvent, just like in high school chemistry, iodine can dissolve in alcohol, benzene, carbon tetrachloride is the same. It has a similar structure and is prone to phase dissolution. In fact, you can rub a coat of nail polish on top of the nail polish and wipe it off with a paper.
How to remove the nail correctly First of all, we must choose regular manufacturers, mild and non-stimulating nail polish remover, preferably without acetone. Secondly, the amount of nail polish remover must be small. Especially when nail polish remover contains acetone, it will make the surface of the nail cuticle due to dry and become rough and fragile. When cleaning nail polish, simply saturate the cotton pad with nail polish remover in an area the size of a nail nail. Third, nail polish remover should not be used to swipe your nails. Especially those who wash nail effect is more significant products, use it to wipe the nail surface, will make the nail surface become dim, dull. Instead, press a cotton pad dampened with nail polish remover against your nails for 10 seconds, then gently rub it down from top to bottom, and the polish is mostly gone. If not, you can do it again.
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