Human EB virus detection plate (EB) ELISA test kit instruction manual

**Human Epstein-Barr Virus Detection Plate (EB) Enzyme-Linked Immunosorbent Assay (ELISA) Kit Instruction Manual** This ELISA kit is intended for research purposes only. It is designed to detect the presence of the Epstein-Barr Virus (EBV) detection plate (EB) in human biological samples such as serum, plasma, cell culture supernatants, and other related liquid specimens. This test supports the identification and hematological analysis of individuals infected with EBV. **Principle of the Test:** The assay is based on a double-antibody sandwich ELISA technique. A microplate is pre-coated with purified anti-EB antibody, which binds specifically to the EB antigen present in the sample. After washing to remove unbound components, an HRP-conjugated secondary antibody is added to form an antibody-antigen-enzyme complex. Following another wash, TMB substrate is added, leading to a color change from blue to yellow under the action of the HRP enzyme and an acidic stop solution. The optical density (OD) at 450 nm is measured using a microplate reader. The results are compared against a cutoff value to determine the presence or absence of EBV in the sample. **Kit Components:** - 48-well configuration: 1 × 48 coated plate, 2 sealing films, 1 sealed bag, 1 negative control (0.5 ml), 1 positive control (0.5 ml), 1 enzyme-labeled reagent (3 ml), 1 sample diluent (3 ml), 1 developer A (3 ml), 1 developer B (3 ml), 1 stop solution (3 ml), 1 concentrated washing solution (20 ml × 20 times). - 96-well configuration: 1 × 96 coated plate, 2 sealing films, 1 sealed bag, 1 negative control (0.5 ml), 1 positive control (0.5 ml), 1 enzyme-labeled reagent (6 ml), 1 sample diluent (6 ml), 1 developer A (6 ml), 1 developer B (6 ml), 1 stop solution (6 ml), 1 concentrated washing solution (20 ml × 30 times). **Storage Instructions:** All components should be stored at 2–8°C. Avoid freezing unless specified. Keep the kit away from light and moisture. **Sample Preparation and Requirements:** - **Serum:** Collect blood at room temperature, allow it to clot for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant. If precipitation occurs, centrifuge again. - **Plasma:** Use EDTA or sodium citrate as anticoagulant. Mix for 10–20 minutes, centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully. - **Urine:** Collect using a sterile container, centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant. - **Cell Culture Supernatant:** Centrifuge at 2000–3000 rpm for 20 minutes. For intracellular components, lyse cells by repeated freeze-thaw cycles and centrifuge again. - **Tissue Samples:** Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge. Store the supernatant at 2–8°C. - **General Note:** Samples should be processed as soon as possible. If not tested immediately, store at -20°C but avoid repeated freezing and thawing. Do not use samples containing NaN₃, as it inhibits HRP activity. **Procedure Steps:** 1. Label the microwells according to the sample type. Set up 2 negative controls, 2 positive controls, and 1 blank control per plate. 2. Add 50 µL of negative and positive control, 40 µL of sample diluent, and 10 µL of sample into the respective wells. 3. Seal the plate and incubate at 37°C for 30 minutes. 4. Prepare the washing solution by diluting the concentrated washing solution with distilled water (1:20 ratio). 5. Wash the plate 5 times with the washing solution, discarding the liquid after 30 seconds each time. 6. Add 50 µL of enzyme-labeled reagent to all wells except the blank. 7. Incubate at 37°C for 30 minutes. 8. Repeat the washing step. 9. Add 50 µL of developer A followed by 50 µL of developer B. Incubate at 37°C for 15 minutes. 10. Add 50 µL of stop solution to terminate the reaction. 11. Measure the OD at 450 nm within 15 minutes of adding the stop solution. **Result Interpretation:** - **Positive Control Mean ≥ 1.00** - **Negative Control Mean ≤ 0.10** - **Cutoff Value = Negative Control Mean + 0.15** - **Sample Result < Cutoff = Negative** - **Sample Result ≥ Cutoff = Positive** **Note:** Ensure all steps are performed carefully and in accordance with the manufacturer’s instructions. Proper handling and storage of samples and reagents are essential for accurate results. **Download:** [Human EB Virus Detection Plate (EB) ELISA Kit Instruction Manual](#) **Author:** [Shanghai Kamaishu Laboratory Research Reagents Procurement Network](#) **Keywords:** EBV, ELISA, Human, Detection, Viral, Research, Kit, Protocol **About Us:** China Education Equipment Purchasing Network is dedicated to providing high-quality laboratory products and services. Explore our website to find the latest equipment and reagents for your research needs. **Disclaimer:** All content is for informational purposes only. Unauthorized reproduction is prohibited. Please respect copyright and intellectual property rights. **Follow Us:** Scan the QR code below to stay updated with the latest news and product information. [QR Code Image] [Logo Image] **Education Equipment Purchasing Network** – Stay informed, stay ahead. **Contact Us:** If you have any questions or need assistance, feel free to reach out. We are here to help.

Wireless Electric Dermapen

Wireless electric dermapen,professional derma stamp pen,chargeable Dr.Pen,micro needling pen,automatic derma stamp

Wireless electric dermapen,professional derma stamp pen,chargeable Dr.Pen,micro needling pen,automatic derma stamp

Guangzhou Vantee Electronic Technology Co., Ltd. , https://www.finerroller.com