Determination of canine adrenomedullin elisa technique
2025-10-02 23:59:40
Canine adrenomedullin ELISA technology test manufacturers not only provide ELISA kits but also offer a wide range of related products, including immunohistochemistry kits, reagents, antibodies, and standards. Our kits are known for high repeatability and reliability, ensuring accurate and consistent results. When you purchase an ELISA kit, you also receive free reagents, making the process more cost-effective. Our technical support is professional, standardized, and efficient, ensuring that your experiments run smoothly.
Guangrui Bio offers comprehensive and high-quality product services, tailored to meet the needs of researchers. We welcome any inquiries and are here to assist you in selecting the right kit for your study.
**Purpose**: This ELISA kit is designed to quantify adrenomedullin (ADM) levels in canine serum, plasma, and other biological fluids. It provides a reliable method for measuring ADM concentrations, which is essential for research in veterinary medicine and endocrinology.
**Experimental Principle**: The kit employs a double-antibody sandwich ELISA technique. A microtiter plate is pre-coated with purified canine ADM antibodies, forming a solid-phase antibody. After adding the sample, ADM binds to the immobilized antibody. An HRP-conjugated secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following thorough washing, TMB substrate is added, leading to a color change from blue to yellow under the catalytic action of HRP. The intensity of the color is directly proportional to the ADM concentration in the sample. Absorbance is measured at 450 nm using a microplate reader, and the ADM concentration is determined by comparing it to a standard curve.
**Kit Composition**:
- 48-well configuration: 1×48 enzyme-labeled plate, 2 sealing films, 1 standard, 1 standard dilution, 1 enzyme reagent, 1 sample dilution, 1 developer A, 1 developer B, 1 solution, 1 concentrated wash solution.
- 96-well configuration: 1×96 enzyme-labeled plate, 2 sealing films, 1 standard, 1 standard dilution, 1 enzyme reagent, 1 sample dilution, 1 developer A, 1 developer B, 1 solution, 1 concentrated wash solution.
**Storage Instructions**: All components should be stored at 2–8°C. Ensure proper sealing to maintain stability and performance.
**Sample Preparation and Requirements**:
1. **Serum**: Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully. If precipitation occurs, re-centrifuge before use.
2. **Plasma**: Use EDTA or sodium citrate as anticoagulant. Mix well, centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant. Re-centrifuge if precipitate forms.
3. **Urine**: Collect in a sterile tube and centrifuge at 2000–3000 rpm for 20 minutes. Carefully collect the supernatant.
4. **Cell Culture Supernatant**: For secreted components, collect in a sterile tube and centrifuge. For intracellular components, lyse cells by freezing and thawing, then centrifuge again.
5. **Tissue Samples**: Homogenize in PBS (pH 7.4), centrifuge, and collect the supernatant. Store the remaining portion at 2–8°C or freeze for later use.
6. **General Handling**: Process samples immediately after collection. If not tested right away, store at -20°C and avoid repeated freeze-thaw cycles.
7. **Note**: Samples containing NaN3 cannot be used, as it inhibits HRP activity.
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Q: What is the MOQ?
A: No MOQ required normally, that means you can always ask for a sample first. But remember the sample freight is at your own cost.
Q: Do I have to have a design to inquire?
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