An overview of cutting-edge technologies for cell culture
In an article in the latest issue of "Nature" magazine, as the technical editor of "Nature" and "Nature Methods" Fei Wenli `Marx (Vivien Marx) wants readers to introduce the new progress of cell culture, and an overview Cutting-edge technology in this field. The following is the full text:
Advances in cell culture media technology can help scientists gain a deeper understanding of the ingredients in these mixtures and what natural environment cells prefer—even in the laboratory.
Cells can thrive in the laboratory, which will undoubtedly relieve many researchers, and vice versa, if cells cannot grow normally even in the correct nutrient medium, this will stop the experiment.
This is why cell culture can bring such an active market. However, although the generation of cell culture research has accumulated several generations of experience, but to make the right choice, it is still more like an art, Not science.
And even if there is a slight difference in the medium, the leaves will have a great impact-often people do not know the reason. Many scientists have their own medium formulations, but this hinders the reproducibility of scientific discoveries.
John Masters, an experimental pathologist from University College London, has edited a lot of data on animal and human cell culture. He said that this "family secret" has many ingredients, and the purity and concentration of the supplier's products vary. The same supplier Different batches of commodities are different, and the inconsistency of chemical compounds is often difficult to repeat.
But as scientists have increasingly recognized the importance of cell culture, this research area has become more rigorous. For example, some researchers are trying to remove the effects of cell culture components from sourced animals, mainly because of concerns. The cell culture is contaminated. Researchers are also trying to make the cell growth environment reproduce the natural situation, such as three-dimensional cell culture.
iPS cell culture
In the "Cell" article published by Yamanaka Yamanaka that year, the cultivation method of iPS cells was pointed out. For this particular cell, the cultivation method is especially important. Stem cells, by cultivating iPS cells, they eventually cultivated multiple tissues in chimeric mice. One of the research groups also proved that these cells can also differentiate into germ cells, so suspension culture reprogramming has been shown to be produced in mice Pluripotent stem cells.
Secondly, in response to the recent transdifferentiation technology, researchers at Harvard University recapitulated the "Directed Differentiation of Pluripotent Stem Cells" snapshot article, which outlines the method of transdifferentiation of pluripotent stem cells into 50 cell types, including each germ layer The cultivation conditions and key growth factors needed, this guide article will help researchers in the field of stem cell research to shorten the time to explore the conditions, undoubtedly quite collectible.
Three-dimensional cell culture
There is increasing evidence that 3D cell culture is closer to the in vivo environment than traditional 2D cell culture. Drug or biological research based on 2D cell culture may be biased, and 3D cell culture can provide us with more realistic information and reduce the time and cost of drug development.
Therefore, related achievements are endless. Researchers from Tsinghua University in China published an article introducing the construction of a 3D microhydrogel that can capture and culture individual neurons for long-term monitoring. In another article, a group of researchers introduced that single cells can be attached to glass through an oligonucleotide membrane. This method can not only realize long-term monitoring of cells, but also increase the possibility of studying the interaction between cells.
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