Human SDF-1β ELISA Kit

**Human SDF-1β ELISA Kit – For the Quantitative In Vitro Determination of Human Stromal Cell-Derived Factor 1β in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Biological Fluids** *For Laboratory Research Use Only. Not for Diagnostic or Therapeutic Purposes.* This ELISA kit is designed for the quantitative measurement of Human SDF-1β in various biological samples. The assay is based on a sandwich immunoassay principle using specific antibodies to detect and quantify the target protein. A standard curve is generated using known concentrations of SDF-1β, allowing for accurate determination of unknown sample levels. The reaction is terminated by adding a stop solution, which changes the color from blue to yellow. The absorbance is then measured at 450 nm using a microplate reader. By comparing the optical density (OD) of the samples to the standard curve, the concentration of SDF-1β can be precisely calculated. --- **Sample Collection and Storage** - **Serum**: Use a serum separator tube. Allow clotting for 2 hours at room temperature or overnight at 4°C. Centrifuge at 2000×g for 20 minutes. Store aliquots at -20°C; avoid repeated freeze-thaw cycles. - **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g, 2–8°C. Store at -20°C. - **Tissue Homogenate, Cell Culture Supernatants, and Other Fluids**: Centrifuge to remove particulates. Assay immediately or store at -20°C. Ensure no hemolysis or granules are present. --- **Materials Required but Not Supplied** - 37°C incubator - Microplate reader capable of measuring absorbance at 450 nm - Precision pipettes, disposable tips, and absorbent paper - Distilled or deionized water --- **Reagents Provided** - Microtiter Strip Plate (12 × 8 strips / 12 × 4 strips) - Standard (6 vials, 0.5 ml/vial) - Sample Diluent (6.0 ml / 3.0 ml) - HRP-Conjugate Reagent (10.0 ml / 5.0 ml) - 20X Wash Solution (25 ml / 15 ml) - Chromogen Solution A (6.0 ml / 3.0 ml) - Chromogen Solution B (6.0 ml / 3.0 ml) - Stop Solution (6.0 ml / 3.0 ml) - Closure Plate Membrane (2 / 2) - User Manual (1 / 1) - Sealed Bags (1 / 1) *Note: Standard concentrations are 8, 4, 2, 1, 0.5, and 0.25 ng/mL. If sample values exceed the highest standard, dilute with Sample Diluent and repeat the assay.* --- **Precautions** - Do not mix reagents from different kits. - Allow all reagents to reach room temperature (20–25°C) before use. - Do not use beyond expiration date. - Use only deionized water. - Keep unused strips in the sealed bag with desiccant. - Wear gloves during the procedure. - Dispose of waste properly after inactivation for 30 minutes. - Avoid contamination of substrate solutions. - Chromogen B contains 20% acetone—keep away from heat or flame. --- **Reagent Preparation** - **Wash Solution (1X)**: Dilute 1 volume of 20X Wash Solution with 19 volumes of deionized or distilled water. Store at 2–8°C for 1 month. --- **Assay Procedure** 1. Prepare standards and samples in duplicate. 2. Add 50 μl of standard or sample to each well (blank well receives nothing). 3. Add 100 μl of HRP-conjugate reagent to all wells except the blank. Incubate for 60 minutes at 37°C. 4. Wash the plate 4 times manually or using an automated washer. 5. Add 50 μl of Chromogen A and 50 μl of Chromogen B. Incubate for 15 minutes at 37°C, protected from light. 6. Add 50 μl of Stop Solution. The color should turn yellow. Mix gently if uneven. 7. Measure OD at 450 nm. Subtract blank OD from all readings. --- **Data Analysis** Plot the average OD values of standards against their concentrations. Determine sample concentrations by interpolation on the standard curve. Each user should generate their own curve due to possible variations in technique or environmental conditions. --- **Performance Characteristics** - Intra-assay and Inter-assay CV < 15%. - Assay range: 0.25 ng/mL – 8 ng/mL. - Sensitivity: < 0.1 ng/mL. - Cross-reactivity: No significant interference observed. - Storage: 2–8°C (frequent use); 6 months at -20°C. **Important Note:** This kit is intended for research purposes only and must not be used in diagnostic procedures. Always follow good laboratory practices when handling biological samples.

Study Room Furniture

ergonomic desk,adjustable chair,study room furniture,home office furniture,desk with storage, comfortable study chair, modern study furniture, educational furniture space,saving study desk ,study room organizatio

Foshan Beausimpe Furniture Co., Ltd , https://www.minottiitaly.com