Description of the total complement (CH50) ELISA test kit
2021-03-16 03:20:36
Total Complement (CH50) ELISA Test Kit Human Description I. Detection Principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with human total complement (CH50) capture antigen, the specimen, standard, and HRP-labeled detection antibody were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the total human complement (CH50) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration.
Beijing Jiehui Biotechnology's most professional ELISA kit supplier is available from stock to ensure the experimental results. Invalid can be returned free of charge, free test service is available, welcome to inquire! 010-57269780!
Second, self-provided items
1. Microplate reader (450nm)
2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
3.37 ° C incubator
4.20× Washing buffer dilution: Distilled water was diluted 1:20, ie 1 part of 20× washing buffer plus 19 parts of distilled water.
5. Manually wash the plate: Drain the liquid in the hole, fill each hole with the washing liquid, let stand for 1 min, then drain the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times.
Automatic washing machine: Inject 350 μL of washing solution into each well, soak for 1 min, and wash the plate 5 times.
Third, the operation steps
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard hole, sample hole and blank hole, blank hole is added; standard product hole is added with different concentration of standard product 50μL;
3. The sample hole to be tested is first added with 10 μL of the sample to be tested, and then 40 μL of the sample diluent is added;
4. Then add 50 μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard well and sample well (without blank). Seal the well with a sealing membrane, 37 ° C water bath or incubator temperature Breed for 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark.
7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Fourth, more details in Beijing Jiehui Biotechnology Co., Ltd. (010-57269780.) Welcome your inquiry and order!
Latest product sales:
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Beijing Jiehui Biotechnology's most professional ELISA kit supplier is available from stock to ensure the experimental results. Invalid can be returned free of charge, free test service is available, welcome to inquire! 010-57269780!
Second, self-provided items
1. Microplate reader (450nm)
2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
3.37 ° C incubator
4.20× Washing buffer dilution: Distilled water was diluted 1:20, ie 1 part of 20× washing buffer plus 19 parts of distilled water.
5. Manually wash the plate: Drain the liquid in the hole, fill each hole with the washing liquid, let stand for 1 min, then drain the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times.
Automatic washing machine: Inject 350 μL of washing solution into each well, soak for 1 min, and wash the plate 5 times.
Third, the operation steps
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard hole, sample hole and blank hole, blank hole is added; standard product hole is added with different concentration of standard product 50μL;
3. The sample hole to be tested is first added with 10 μL of the sample to be tested, and then 40 μL of the sample diluent is added;
4. Then add 50 μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard well and sample well (without blank). Seal the well with a sealing membrane, 37 ° C water bath or incubator temperature Breed for 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark.
7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Fourth, more details in Beijing Jiehui Biotechnology Co., Ltd. (010-57269780.) Welcome your inquiry and order!
Latest product sales:
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