Phenol: chloroform: isoamyl alcohol = 125:24:1 (PH<5.0) Description Download

Phenol: Chloroform: isoamyl alcohol = 125:24:1 (pH < 5.0) Specification: 100ml / 500ml Storage: 4 ° C, protected from light, valid for 12 months. Product Description: This reagent is a mixture of water-saturated phenol, chloroform and isoamyl alcohol in a volume ratio of 125:24:1. It is mainly used for the extraction of RNA. Phenol denatures proteins while inhibiting the degradation of RNase. The chloroform extraction accelerates the stratification of the organic phase and the liquid phase to remove trace phenols from the nucleic acid solution. Isoamyl alcohol reduces air bubbles generated during protein denaturation operations. Using phenol:chloroform: isoamyl alcohol extraction, the upper layer is an RNA-containing aqueous phase, the middle is a denatured protein and DNA, and the lower layer is an organic solvent phase. After standing, the product has a stratification phenomenon, and the upper layer is transparent to the aqueous phase, and the lower organic phase can be taken up during use. Note: This reagent is highly corrosive and should be avoided as far as possible to avoid skin contact or inhalation. If it turns red or brown, it indicates that oxidation has occurred and cannot be used any more.

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