New era of application of density gradient overspeed separation technology
Ultracentrifugation is an important method in current life science research, and density gradient ultracentrifugation has become one of the important methods for separating and purifying biological samples, such as viruses, subcellular organelles, proteins, and ribosomes. However, since the preparation of the density gradient is mostly a layer method or a freeze-thaw method, the gradient is nonlinear and the separation effect is poor. The sample collection process is still in the manual stage, which takes a long time, and the repeatability and separation accuracy are poor, which limits the application of the density gradient method.
The automatic density gradient preparation and separation system developed by Biocomp has effectively solved this problem. The company was founded in 1985 and was founded by David Coombs, Ph.D. R & D and production of systems related to life science research. At present, it has many internationally renowned users, such as Harvard University, Yale University, University of Pennsylvania, Tsinghua University, Institute of Biophysics, Chinese Academy of Sciences, Institute of Genetic Development, etc.
The automatic density gradient preparation system has a number of different gradient programs built-in, users can choose according to their own needs, and can also set the speed, time and angle of gradient preparation by themselves, and can connect to a computer to upload and download programs. Gradient media are available, such as Soucrose, CsCl, NaCl, Glycerol, Ficoll, etc.
Take the density gradient of 5% -20% sucrose solution as an example: just add 5% and 20% of the maximum and minimum concentration solutions to the centrifuge tube in 1: 1 volume; put it into the gradient preparator and select the corresponding program to rotate and centrifuge. To obtain a completely linear density gradient, the fastest can be completed 6 / min.
The automatic density gradient separation and collection system adopts a patented trumpet-shaped suction head to suck the sample strip layer by layer under the drive of the motor, which effectively reduces the disturbance and strip confusion generated during the sample separation process, without dispersing laminar flow and tailing. .
The motor control speed is 0.2-6.5mm / sec, the resolution can reach 10um, so that the separation accuracy can reach 99%, and the repeatability is 0.01mm.
With automatic cleaning function, the pipeline can be automatically set or manually cleaned and dried to effectively avoid cross-contamination.
The software system connects the instrument to the computer, displays the sample collection process in real time, and draws the UV absorption spectrum. All the data has been excel output for easy data analysis.
Application field Biocomp automatic density gradient preparation and separation system has been widely used, mainly for the separation of various biological samples such as viruses, subcellular organelles, proteins, ribosomes, nucleic acids, vesicles and so on. Several articles applying this product have been published in internationally renowned journals such as Nature, Science, PNAS, Plant Cell, etc. In 2006, Northwestern University also used this product to successfully separate carbon nanotube materials, published in Nature Nanotecnology.
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